Nanny Nane (voiced by Jane Carr), is the main villain in Alvin and the Chipmunks 3: Nursery Crimes who is secretly the infamous Japanese British female serial killer known as Nina Nakamura. Nanny Nane has the boyfriend of her associate Eleanor Miller (Amy Poehler), named Theodore Seville (Jesse McCartney), kidnapped by her right-hand woman Camille Leon (Ashley Tisdale) so she can hold him for ransom, whilst pretending to help Eleanor Miller to find him as she awaits for the ransom money. Simon Seville (Matthew Gray Gubler) and his older brother Alvin Seville (Justin Long) are assigned the task of locating and rescuing their younger brother Theodore, as well as discovering the identities of his attackers.
Nanny Nane appears at the Osaka Convention Center where a precious and expensive Japanese artifacts are on display, and she is seen making a speech with Eleanor, Brittany (Christina Applegate), and Jeanette (Anna Faris) at her side. Both Alvin and Simon arrives and Nanny Nane's cover is soon exposed and blown, and she reveals that Theodore is being held hostage in a dark blue Toyota van outside with an explosive black leather jacket attached to him. Alvin goes to rescue Theodore and dares Nanny Nane to detonate the explosive black leather jacket in front of her and Camille Leon (who is also present). Nanny Nane escapes into the and Simon pursues her, having successfully removed the explosive black leather jacket from Theodore, while Alvin (along with Brittany and Jeanette) deals with Nanny Nane's right-hand woman Camille Leon and finally kills her in revenge. Enraged, Nanny Nane attacks and hits Simon with her snakeskin briefcase full of ransom money, but accidentally topples over the edge, and is left hanging onto Simon's explosive black leather jacket. As she struggles to keep her grips, the explosive black leather jacket rips in half and sends Nanny Nane plunging/falling to her death onto the roof of a parked silver Honda van. She made a cameo appearance as one of Theodore Seville's queen cards alongside Shego, Adrena Lynn, Bonnie Rockwaller, Connie Rockwaller, Loonie Rockwaller, Warmonga, Camille Leon, Electronique, and DNAmy in Alvin and the Chipmunks 3: The Twilight Samurai Assassins.